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Homo sapiens
Homo sapiens
Mus musculus
Homo sapiens
Mus musculus
Homo sapiens
Mus musculus
Mus musculus
Homo sapiens
Mus musculus
Transcription Factor Encyclopedia  BETA
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At the present 3 different translation products were related to 4 NR2F2 transcripts: (a) Isoform a is encoded by transcript variant 1 (the longest) and constitutes the full length protein; (b) Isoform b is encoded by transcript variant 2, has a shorter N-Terminus when comparing to isoform a (lacks A/B domain and DBD); and (c) Isoform c is encoded by transcript variants 3 and 4, has a shorter N-Terminus when comparing to isoform a and b (lacks "A/B, DBD and few residues of the D domain). Figure 2 (summary section) illustrate the regular transcript and figures 4 (summary section), 5 and 6 (structure section) illustrate the NR2F2's multidomain structure.

The C-terminus is identical for all forms from residue 154 of isoform a. These isoforms and their sequences can be accessed at Entrez Gene (GeneID: 7026). Thus, all the isoforms includes the LBD and isoforms b and c could play a key role on regulation NR2F2 function. Indeed, in human embryonic stem cells, shorter variants have been associated to enhanced activity of the long isoform[1]. Nevertheless, the available antibodies against COUP-TFII are not raised against the region common to all isoforms, so, the presence of the proteins corresponding to isoforms b and c were never demonstrated.

NR2F2 related antibodies include: (1) a NR2F2 specific mice monoclonal antibody validated for IF, IP, gel shift (R&D Systems, PP-H7147-00), and (2) a NR2F2 and NR2F1 specific rabbit polyclonal antibody validated for IP, IF, gel shift and western blot [2].

  1. Rosa A and Brivanlou AH. A regulatory circuitry comprised of miR-302 and the transcription factors OCT4 and NR2F2 regulates human embryonic stem cell differentiation. EMBO J., 30(2):237-48. (PMID 21151097)
  1. Lou DQ et al. Chicken ovalbumin upstream promoter-transcription factor II, a new partner of the glucose response element of the L-type pyruvate kinase gene, acts as an inhibitor of the glucose response. J. Biol. Chem., 274(40):28385-94. (PMID 10497199)
Covalent modifications

List of potential covalent modifications found by Prosite:

PS00005 PKC_PHOSPHO_SITE Protein kinase C phosphorylation site

7 - 9: TwR

68 - 70: SdK

87 - 89: SgK

106 - 108: SvR

114 - 116: TcR

193 - 195: TsR

369 - 371: SlR

PS00006 CK2_PHOSPHO_SITE Casein kinase II phosphorylation site

7 - 10: TwrD

167 - 170: TngD

222 - 225: SavE

248 - 251: TwsE

336 - 339: SlqE

376 - 379: SviE

390 - 393: TpiE

PS00008 MYRISTYL N-myristoylation site

17 - 22: GSqgSQ

20 - 25: GSqaSQ

46 - 51: GGpaST

64 - 69: GGpgSD

92 - 97: GQftCE

403 - 408: GSsfNW

PS00001 ASN_GLYCOSYLATION N-glycosylation site

110 - 113: NLSY

List of potential covalent modifications found by manually

PS00004 CAMP_PHOSPHO_SITE cAMP- and cGMP-dependent protein kinase phosphorylation site

108 - 112: RRNLS