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 SOX9
Homo sapiens
 HIF1A
Homo sapiens
 Pax6
Mus musculus
 PAX6
Homo sapiens
 Snai2
Mus musculus
 PPARA
Homo sapiens
 Ppara
Mus musculus
 Thrb
Mus musculus
 SNAI2
Homo sapiens
 Tbr1
Mus musculus
Transcription Factor Encyclopedia  BETA
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Isoforms

At the present 3 different translation products were related to 4 NR2F2 transcripts: (a) Isoform a is encoded by transcript variant 1 (the longest) and constitutes the full length protein; (b) Isoform b is encoded by transcript variant 2, has a shorter N-Terminus when comparing to isoform a (lacks A/B domain and DBD); and (c) Isoform c is encoded by transcript variants 3 and 4, has a shorter N-Terminus when comparing to isoform a and b (lacks "A/B, DBD and few residues of the D domain). Figure 2 (summary section) illustrate the regular transcript and figures 4 (summary section), 5 and 6 (structure section) illustrate the NR2F2's multidomain structure.

The C-terminus is identical for all forms from residue 154 of isoform a. These isoforms and their sequences can be accessed at Entrez Gene (GeneID: 7026). Thus, all the isoforms includes the LBD and isoforms b and c could play a key role on regulation NR2F2 function. Indeed, in human embryonic stem cells, shorter variants have been associated to enhanced activity of the long isoform[1]. Nevertheless, the available antibodies against COUP-TFII are not raised against the region common to all isoforms, so, the presence of the proteins corresponding to isoforms b and c were never demonstrated.

NR2F2 related antibodies include: (1) a NR2F2 specific mice monoclonal antibody validated for IF, IP, gel shift (R&D Systems, PP-H7147-00), and (2) a NR2F2 and NR2F1 specific rabbit polyclonal antibody validated for IP, IF, gel shift and western blot [2].

References
  1. Rosa A and Brivanlou AH. A regulatory circuitry comprised of miR-302 and the transcription factors OCT4 and NR2F2 regulates human embryonic stem cell differentiation. EMBO J., 30(2):237-48. (PMID 21151097)
  1. Lou DQ et al. Chicken ovalbumin upstream promoter-transcription factor II, a new partner of the glucose response element of the L-type pyruvate kinase gene, acts as an inhibitor of the glucose response. J. Biol. Chem., 274(40):28385-94. (PMID 10497199)
Covalent modifications

List of potential covalent modifications found by Prosite:


PS00005 PKC_PHOSPHO_SITE Protein kinase C phosphorylation site

7 - 9: TwR

68 - 70: SdK

87 - 89: SgK

106 - 108: SvR

114 - 116: TcR

193 - 195: TsR

369 - 371: SlR


PS00006 CK2_PHOSPHO_SITE Casein kinase II phosphorylation site

7 - 10: TwrD

167 - 170: TngD

222 - 225: SavE

248 - 251: TwsE

336 - 339: SlqE

376 - 379: SviE

390 - 393: TpiE


PS00008 MYRISTYL N-myristoylation site

17 - 22: GSqgSQ

20 - 25: GSqaSQ

46 - 51: GGpaST

64 - 69: GGpgSD

92 - 97: GQftCE

403 - 408: GSsfNW


PS00001 ASN_GLYCOSYLATION N-glycosylation site

110 - 113: NLSY


List of potential covalent modifications found by manually


PS00004 CAMP_PHOSPHO_SITE cAMP- and cGMP-dependent protein kinase phosphorylation site

108 - 112: RRNLS